Module 4: Measuring Biodiversity

These free OCR A Level Biology Measuring Biodiversity revision notes have been written for specification points 4.2.1(b.i), 4.2.1(c) and 4.2.1(d).

Measuring Biodiversity

Sampling techniques allow for a reliable estimation of biodiversity to be made by collecting data on which species are present in an area and their abundance

The table below outlines the different sampling methods:

Sampling MethodDescriptionAdvantageDisadvantage
RandomSites are chosen randomly.Removes bias.Species may be missed.
OpportunisticLocations are chosen based on availability, accessibility or knowledge.Produces data quickly.Inherently biased, making the data highly unreliable.
StratifiedThe habitat is divided into areas (strata) based on their differences, and each is sampled.All different areas of a habitat are sampled.Some areas may be smaller, overrepresenting some species.
SystematicSamples are taken at regular intervals along a measurable abiotic gradient.Provides paired data: The location and abundance of species sampled is linked with measured abiotic factors.Species outside of the transect are missed.
Field equipment:

Several standard techniques are used to collect data on different organisms:

  • Pitfall traps: Containers sunk into the ground to catch small ground-dwelling animals.
  • Pooters: Suck up small invertebrates into a container without harm.
  • Quadrats: Square frames (e.g. 0.5 m × 0.5 m or 1 m × 1 m) used to estimate plant abundance. Quadrats are laid randomly or at intervals along a transect. Species may be counted directly or estimated by percentage cover.
  • Sweep nets: Used for catching insects in tall vegetation.
  • Transects: Lines across the habitat. A line transect records species touching the line; a belt transect involves placing quadrats along the line.

Measuring Species Richness and Species Evenness

Biodiversity within a habitat depends not only on the number of different species present, but also on how evenly the individuals are distributed among those species.

For example, the table below compares the abundance of 20 species in two habitats: 

HabitatSpecies PresentRichnessEvennessInterpretation
Wildflower meadow20 species, each with similar abundance.HighHighHigh biodiversity: many species with balanced populations.
Managed lawn20 species, dominated by 2 species.HighLowLower biodiversity: same richness but reduced evenness.
Simpson’s Index of Diversity

Simpson’s Index of Diversity (D) provides a quantitative measure of biodiversity that takes both species richness and evenness into account. 

It can be used for comparing different habitats or monitoring changes over time.

The Formula

D = 1 – ∑ (n / N)²

Where:

  • n = number of individuals of a particular species
  • N = total number of individuals of all species
  • = the sum of
  • D = a value between 0 and 1.

Interpreting D

A high D (closer to 1) means high biodiversity; many species and/or even populations.
A low D (closer to 0) means low biodiversity; few species and/or dominated by one.


Example Calculation

Suppose a habitat has the following species counts:

SpeciesNumber of Individuals (n)
Buttercup25
Dandelion25
Daisy25
Dock25
Total (N)100

Using the formula:

D = 1 − ∑ (n / N)²

For convenience, data is typically processed in the following format:

Speciesnn/N(n/N)²
Dandelion850.850.7225
Buttercup50.050.0025
Daisy50.050.0025
Dock50.050.0025
Sum (∑)0.73
D = 1 − ∑0.27

This low Simpsons Index of Diversity value (D) indicates low species evenness and biodiversity.

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